NCI-H520-CAS9 cell line

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NCI-H520-CAS9 Order

Catalog#

YC-C017-Cas9-H

Size

1*10^6

Transfection Culture Medium

Transfection Culture Medium-D

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Datasheet

Order

Detailed Product Information

Product category

Cas9 Stable Cell Lines

cell name

NCI-H520-CAS9

Cell Morphology

Epithelial-like, adherent

Passage ratio

1:3~1:4

Species

Human

Expression gene

Cas9

Resistance gene

hygro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90%RPMI-1640+10% FBS

Description

Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the NCI-H520 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a NCI-H520-CAS9 cell line that can maintain long-term, stable and high expression.

Product Validation Data（RT-QPCR）

Sample Name	Target Name	Cт Mean	ΔCт
NCI-H520-CAS9	Cas9	24.85734749	4.89374161
NCI-H520-CAS9	β-actin	19.96360588	4.89374161
NCI-H520	Cas9	31.09050941	12.19707679
NCI-H520	β-actin	18.89343262	12.19707679

Cutting Efficiency Validation

NCI-H520-CAS9 cell line Product Validation Data（RT-QPCR）

Note: The above figure shows the sequencing peaks of the NCI-H520-CAS9 stable cell pool which is electroporated by RP11-423H2.3 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:NCI-H520			STR Info (Cell bank) Cell Line:NCI-H520 [H520]
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	12	13		12	13
D13S317	10	11		10	11
D7S820	8	12		8	12
D16S539	8	13		8	13
VWA	18	19		18	19
TH01	10	10		10	10
AMEL	X	X		X	X
TPOX	8	8		8	8
CSF1PO	10	10		10	10
D12S391	21	21
FGA	22	22
D2S1338	18	23
D21S11	30	30
D18S51	17	17
D8S1179	14	16	17.0
D3S1358	16	16
D6S1043	12	18
PENTAE	5	14
D19S433	13	14
PENTAD	13	13
D1S1656	14	16.3

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
01
High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
02
High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
03

NCI-H520-CAS9

Catalog#

YC-C017-Cas9-H

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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NCI-H520-CAS9 cell line

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